GPR17 Antibody (CL10474) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44216

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10474

Format

Azide and BSA Free
View all GPR17 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q13304, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GPR17 Antibody (CL10474) - Azide and BSA Free

GPR17 Antibody (CL10474) - Azide and BSA Free Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Analysis in human cerebral cortex and kidney tissues using NBP3-44216 antibody. Corresponding GPR17 RNA-seq data are presented for the same tissues.
GPR17 Antibody (CL10474) - Azide and BSA Free Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Staining of human kidney shows no positivity in cells in tubules as expected.
GPR17 Antibody (CL10474) - Azide and BSA Free Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of oligodendrocytes.
GPR17 Antibody (CL10474) - Azide and BSA Free Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
GPR17 Antibody (CL10474) - Azide and BSA Free Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Immunohistochemistry-Paraffin: GPR17 Antibody [NBP3-44216]

Staining of human prostate shows no positivity in glandular cells as expected.
GPR17 Antibody (CL10474) - Azide and BSA Free Western Blot: GPR17 Antibody [NBP3-44216]

Western Blot: GPR17 Antibody [NBP3-44216]

Analysis in human cell line U-251 MG.

Applications for GPR17 Antibody (CL10474) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR17

GPR17, a former orphan G-coupled protein is specifically activated by P2Y and CysLT endogenous ligands, leading to both adenylyl cyclase inhibition and intracellular calcium increases. In both rats and humans GPR17 expressed in the organs which suffer ischemic damage, like the brain, heart and kidneys. Studies by Ciana, et. al. have shown that inhibition of GPR17 reduces ischemic damage in a rat focal ischemia model. These results suggest GPR17 is the common molecular target mediating brain damage by nucleotides and CysLTs.

Long Name

G Protein-coupled Receptor 17

Alternate Names

P2Y-like Receptor, R12, UDP/CysLT Receptor

Gene Symbol

GPR17

Additional GPR17 Products

Product Documents for GPR17 Antibody (CL10474) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GPR17 Antibody (CL10474) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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