GPR17 Antibody

Novus Biologicals | Catalog # NBP1-51988

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GPR17 Primary Antibodies »

Product Specifications

Immunogen

This GPR17 Antibody was develped against a peptide with sequence C-EGKTNESSLSAKSE corresponding to C-Terminus according to NP_005282.1.

Epitope

C-EGKTNESSLSAKSE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GPR17 Antibody

Western Blot: GPR17 Antibody [NBP1-51988]

Western Blot: GPR17 Antibody [NBP1-51988]

Western Blot: GPR17 Antibody [NBP1-51988] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer) with antibody at 3 ug/mL. Detected by chemiluminescence. Approx 40 kDa band observed in Mouse Brain lysates (calculated MW of 37.8kDa according to Mouse NP_001020552.1 ).
Immunocytochemistry/ Immunofluorescence: GPR17 Antibody [NBP1-51988]

Immunocytochemistry/ Immunofluorescence: GPR17 Antibody [NBP1-51988]

Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NBP1-51988] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: GPR17 Antibody [NBP1-51988]

Immunocytochemistry/ Immunofluorescence: GPR17 Antibody [NBP1-51988]

Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NBP1-51988] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for GPR17 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

2 - 3 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GPR17

GPR17, a former orphan G-coupled protein is specifically activated by P2Y and CysLT endogenous ligands, leading to both adenylyl cyclase inhibition and intracellular calcium increases. In both rats and humans GPR17 expressed in the organs which suffer ischemic damage, like the brain, heart and kidneys. Studies by Ciana, et. al. have shown that inhibition of GPR17 reduces ischemic damage in a rat focal ischemia model. These results suggest GPR17 is the common molecular target mediating brain damage by nucleotides and CysLTs.

Long Name

G Protein-coupled Receptor 17

Alternate Names

P2Y-like Receptor, R12, UDP/CysLT Receptor

Entrez Gene IDs

2840 (Human); 574402 (Mouse); 767613 (Rat)

Gene Symbol

GPR17

UniProt

NP_005282.1 (Human)

Additional GPR17 Products

Product Documents for GPR17 Antibody

Certificate of Analysis

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Product Specific Notices for GPR17 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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