GPR55 Antibody - BSA Free

Novus Biologicals | Catalog # NB110-55498

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Canine, Equine

Predicted:

Monkey (95%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPR55 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR55.

Epitope

Third cytoplasmic loop of human

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%), Marmoset (95%), Bovine (85%), Mouse (80%), Equine (80%), Porcine (80%). Canine reactivity reported from a verified customer review.

Specificity

Human GPR55. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPR55 Antibody - BSA Free

Immunohistochemistry: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry: GPR55 Antibody - BSA Free [NB110-55498]

GPR55-Antibody-Immunohistochemistry-NB110-55498-img0005.jpg
Western Blot: GPR55 AntibodyBSA Free [NB110-55498]

Western Blot: GPR55 AntibodyBSA Free [NB110-55498]

GPR55-Antibody-Western-Blot-NB110-55498-img0004.jpg
Immunohistochemistry-Paraffin: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry-Paraffin: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry-Paraffin: GPR55 Antibody [NB110-55498] - Analysis of anti-GPR55 antibody with human pancreas, islet of langerhans.
Immunohistochemistry-Frozen: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry-Frozen: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry-Frozen: GPR55 Antibody [NB110-55498] - Cryosection of canine cervical dorsal root ganglion in which satellite glial cells and some sensory neurons expressed GPR55 immunoreactivity. Image submitted by a verified customer review.
GPR55 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] -

Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GPR55 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] -

Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GPR55 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml
Application Notes
Use in WB reported in scientific literature (PMID:31608295). GPR55 antibody validated for IHC-F from a verified customer review..

Reviewed Applications

Read 1 review rated 5 using NB110-55498 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPR55

G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.

Long Name

G-Protein-Coupled Receptor 55

Alternate Names

Gm218

Entrez Gene IDs

9290 (Human)

Gene Symbol

GPR55

UniProt

Q9Y2T6 (Human)

Additional GPR55 Products

Product Documents for GPR55 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GPR55 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GPR55 Antibody - BSA Free

Customer Reviews for GPR55 Antibody - BSA Free (1)

5 out of 5
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Customer Images

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  • GPR55 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Canine sensory neurons and Canine peripheral sensory neurons
    Species: Canine
    Verified Customer | Posted 07/08/2019
    Cryosection of canine cervical dorsal root ganglion in which satellite glial cells and some sensory neurons expressed GPR55 immunoreactivity.
    GPR55 Antibody - BSA Free NB110-55498

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Protocols

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FAQs for GPR55 Antibody - BSA Free

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  • Q: Do you have any data about the use of the following antibody in Immunofluorescence and Confocal Microscopy:

    A: We have only validated product NB110-55498 for use in IHC Paraffin. Please see our images on the datasheet. The recommended dilution for this antibody in IHC-P is 5 ug/ml.

  • Q: I'm interested in one of your products: GPR55 Antibody (NB110-55498). I'd like to know if it has ever been used in non permeabilised cells, indicating that it can efficiently recognize an extracellular epitope.

    A: We have done validation of this product in IHC-P of pancreas as well as spleen tissues after heat-induced antigen retrieval and yes, permeabilization was executed before blocking/primary antibody incubation step in the protocol. Have you seen our another GPR55 antibody (NLS6817)? This antibody has been generated employing an immunogen which was a synthetic peptide made to the 3rd extracellular domain of GPR55 protein. Because this antibody should technically be detecting extra-cellular domain of the target, you may try skipping permeabilization step for this antibody, however, you will still need to perform antigen retrieval.

  • Q: Do you have any data about the use of the following antibody in Immunofluorescence and Confocal Microscopy:

    A: We have only validated product NB110-55498 for use in IHC Paraffin. Please see our images on the datasheet. The recommended dilution for this antibody in IHC-P is 5 ug/ml.

  • Q: I'm interested in one of your products: GPR55 Antibody (NB110-55498). I'd like to know if it has ever been used in non permeabilised cells, indicating that it can efficiently recognize an extracellular epitope.

    A: We have done validation of this product in IHC-P of pancreas as well as spleen tissues after heat-induced antigen retrieval and yes, permeabilization was executed before blocking/primary antibody incubation step in the protocol. Have you seen our another GPR55 antibody (NLS6817)? This antibody has been generated employing an immunogen which was a synthetic peptide made to the 3rd extracellular domain of GPR55 protein. Because this antibody should technically be detecting extra-cellular domain of the target, you may try skipping permeabilization step for this antibody, however, you will still need to perform antigen retrieval.

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