GPR81 Antibody - BSA Free

Novus Biologicals | Catalog # NLS2095

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPR81 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 19 amino acid peptide from C-terminus of human GPR81.

Epitope

C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (95%). Mouse reactivity reported in the literature (PMID: 19633298).

Localization

Multi-pass membrane protein

Specificity

Human GPR81. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except KRBA1 (53%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPR81 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GPR81 Antibody - BSA Free [NLS2095]

Immunocytochemistry/ Immunofluorescence: GPR81 Antibody - BSA Free [NLS2095]

GPR81-Antibody-Immunocytochemistry-Immunofluorescence-NLS2095-img0010.jpg
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Brain, Substantia Nigra, pigmented and nonpigmented neurons.
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human anterior pituitary.
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human colon, carcinoma.
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Ovary, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human pancreas, carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Breast, Carcinoma
GPR81 Antibody - BSA Free

Western Blot: GPR81 Antibody - BSA Free [NLS2095] -

Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Stable knockdown of GPR81 in MDA-MB-231 cells. Using a lentiviral system, GPR81 was stably knocked down in MDA-MB-231 cells with GPR81-specific short hairpin RNA (shGPR81 #1) & compared with cells containing scrambled shRNA as a control (shNT). Knockdown of GPR81 was confirmed by (a) western blotting & (b) RT-qPCR. The GPR81, MCT1, & MCT4 mRNA levels in the shGPR81 #1 cells are presented as the fold increase compared with those in shNT cells. Data are presented as the means ± s.d. (n = 3/group). **p < 0.01; Student’s t-test. (c) Lactate production in shNT & shGPR81 #1 cells. The cells were cultured for 24 h & lactate concentrations in the supernatants were determined (n = 3/group). **p < 0.01 (vs. shNT cells); Student’s t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GPR81 Antibody - BSA Free

Western Blot: GPR81 Antibody - BSA Free [NLS2095] -

Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GPR81 Antibody - BSA Free

Western Blot: GPR81 Antibody - BSA Free [NLS2095] -

Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GPR81 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 - 20 ug/ml
Application Notes
WB reactivity reported in (PMID: 19633298). ICC/IF reactivity reported in (PMID: 32071396)..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPR81

The receptor FKSG80/GPR81 is a member of the Chemokine Receptor subfamily. FKSG80/GPR81 has been reported primarily in adipose and pituitary. ESTs for FKSG80/GPR81 have been isolated from human bladder cancer and colon cancer libraries.

Long Name

G Protein-coupled Receptor 81

Alternate Names

FKSG80, GPR104, HCA1, HCAR1, LACR1, TA-GPCR

Entrez Gene IDs

27198 (Human)

Gene Symbol

HCAR1

UniProt

Q9BXC0 (Human)

Additional GPR81 Products

Product Documents for GPR81 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GPR81 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GPR81 Antibody - BSA Free

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Protocols

View specific protocols for GPR81 Antibody - BSA Free (NLS2095):

Immunohistochemistry Protocol for GPR81 Antibody (NLS2095):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GPR81 Antibody - BSA Free

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  • Q: We would like to know the Molecular weight of NLS2095.

    A: This antibody was raised to a synthetic 19 amino acid peptide derived from the C-terminus of the human protein. Human GPR81 has a theoretical molecular weight of approximately 39kDa according to the Uniprot accession: Uniprot site

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