GPT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89111

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MASSTGDRSQAVRNGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GPT Antibody - BSA Free

Western Blot: GPT Antibody [NBP1-89111]

Western Blot: GPT Antibody [NBP1-89111]

Western Blot: GPT Antibody [NBP1-89111] - Western blot analysis in mouse liver tissue and rat liver tissue.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Western Blot: GPT Antibody [NBP1-89111]

Western Blot: GPT Antibody [NBP1-89111]

Western Blot: GPT Antibody [NBP1-89111] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human tonsil shows low expression as expected.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human colon.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human kidney.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human liver using Anti-GPT antibody NBP1-89111.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human tonsil using Anti-GPT antibody NBP1-89111.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human colon, kidney, liver and lymph node using Anti-GPT antibody NBP1-89111 (A) shows similar protein distribution across tissues to independent antibody NBP1-89110 (B).
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Analysis in human liver and lymph node tissues using NBP1-89111 antibody. Corresponding GPT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111]

Immunohistochemistry-Paraffin: GPT Antibody [NBP1-89111] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Applications for GPT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPT

GPT, also known as Alanine aminotransferase 1, is a 496 amino acid that is 55 kDa, cytoplasm located, most commonly found in liver, kidney, heart, and skeletal muscles; acts as a catalyzer of the reversible transamination between alanine and 2-oxoglutarate to compose pyruvate and glutamate, is involved in cellular nitrogen metabolism, and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Current research is being performed on several diseases and disorders including vitelliform macular dystrophy, liver disease, epidemic typhus, pyomyositis, kidney cortex necrosis, scrub typhus, hepatitis e, hepatitis c, iron overload hepatitis b, glucose intolerance, biliary atresia, alcohol abuse, cholangitis, cholecystitis, hellp syndrome, viral hepatitis, siderosis, choledocholithiasis, hepatitis a, kawasaki disease, wilson disease, and hepatic encephalopathy. This protein has shown to have interactions with CAPN1, CAPN3, HSPD1, THNSL2, GPT2, AND PSAT1 in pathways such as alanine, aspartate and glutamate metabolism, amino acid synthesis and interconversion (transamination), and metabolism of amino acids and derivatives.

Alternate Names

AAT1alanine aminotransferase 1, ALT1, ALT1EC 2.6.1.2, Glutamate pyruvate transaminase 1, glutamic-alanine transaminase 1, Glutamic--alanine transaminase 1, glutamic-pyruvate transaminase (alanine aminotransferase), Glutamic--pyruvic transaminase 1, GPT1GPT 1

Entrez Gene IDs

2875 (Human)

Gene Symbol

GPT

UniProt

P24298 (Human)

Additional GPT Products

Product Documents for GPT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GPT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GPT Antibody - BSA Free

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Protocols

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FAQs

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