GR/NR3C1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB120-3579

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GR/NR3C1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.

Localization

Cytoplasmic in the absence of ligand; nuclear after ligand-binding.

Specificity

NB120-3579 detects glucocorticoid receptor (GR) from human, mouse and rat tissues.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GR/NR3C1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in A2058 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Applications for GR/NR3C1 Antibody - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1 ug/ml

Immunohistochemistry-Frozen

1 ug/ml

Immunoprecipitation

5 ug/ml

Simple Western

1:10

Western Blot

5 ug/ml
Application Notes
WB: Detects an approx. 97 kDa protein representing GR as well as two other unidentified proteins at approx. 135 - 145 kDa from rat liver extract. IHC: Staining of GR in rat hippocampus using NB120-3579 results in staining primarily in the nucleus. NB120-3579 does not disrupt receptor-DNA interactions.
See Simple Western Antibody Database for Simple Western validation: Tested in Primate Corpus Luteum, separated by Size, antibody dilution of 1:10, apparent MW was 48, 61, 95 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with PBS.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GR/NR3C1

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Entrez Gene IDs

2908 (Human); 14815 (Mouse); 24413 (Rat)

Gene Symbol

NR3C1

UniProt

P04150

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GR/NR3C1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail