Granulin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87324

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Granulin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLL

Reactivity Notes

Mouse reactivity reported in (PMID: 29511098). Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Granulin Antibody - BSA Free

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining in human placenta and skeletal muscle tissues. Corresponding GRN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Granulin Antibody [NBP1-87324]

Immunocytochemistry/ Immunofluorescence: Granulin Antibody [NBP1-87324]

Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87324] - Staining of human cell line A-431 shows localization to endoplasmic reticulum & lysosomes. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human placenta shows moderate to strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in striated muscle fibers.

Applications for Granulin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Granulin

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Alternate Names

acrogranin, GEP, GP88, granulin, granulin-epithelin, granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, progranulin

Gene Symbol

GRN

Additional Granulin Products

Product Documents for Granulin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Granulin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Granulin Antibody - BSA Free

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Protocols

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