GRASP55 Antibody (CL2610) - BSA Free

Novus Biologicals | Catalog # NBP2-36779

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2610

Format

BSA Free
View all GRASP55 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9H8Y8, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for GRASP55 Antibody (CL2610) - BSA Free

Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779]

Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779]

Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779] - analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in U2OS cell line with Anti-GORASP2 monoclonal antibody, showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in human epididymis and skeletal muscle tissues. Corresponding GORASP2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in HeLa cell line showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human cell line A431 showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in MCF7 cell line showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in U251 cell line showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human endometrium shows moderate to strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human epididymis shows strong positivity in glandular cells.
Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779]

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Applications for GRASP55 Antibody (CL2610) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000-1:10000

Immunohistochemistry-Paraffin

1:5000-1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRASP55

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in establishing the stacked structure of the Golgi apparatus. [provided by RefSeq]

Alternate Names

Golgi phosphoprotein 6, golgi reassembly stacking protein 2, 55 kDa, golgi reassembly stacking protein 2, 55kDa, Golgi reassembly-stacking protein of 55 kDa, GOLPH6DKFZp434D156, GRASP55FLJ13139, GRS2Golgi reassembly-stacking protein 2, p59

Gene Symbol

GORASP2

UniProt

Q9H8Y8 (Human)

Additional GRASP55 Products

Product Documents for GRASP55 Antibody (CL2610) - BSA Free

Certificate of Analysis

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Product Specific Notices for GRASP55 Antibody (CL2610) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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