GRP78/HSPA5 Antibody [DyLight 405]

Novus Biologicals | Catalog # NBP1-06274V

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Sheep, Zebrafish

Predicted:

Bovine (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG
View all GRP78/HSPA5 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:34327238) C. elegans reactivity reported in scientific literature (PMID: 31398187).

Localization

Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for GRP78/HSPA5 Antibody [DyLight 405]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry Free-Floating

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GRP78/HSPA5

HSP70 family member GRP78 (78 kD glucose-regulated protein) is localized in ER lumen as well as melanosomes (stage I to stage IV) where it facilitates protein folding/assembly, protein quality control, Ca2+ binding, and regulates ER stress signaling. GRP78 interacts with DNAJC1 and is a component of EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) as well as chaperone multiprotein complex (DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1, ERP29). GRP78 also interacts with TMEM132A as well as TRIM21, and may form a complex with ERLEC1, OS9, SEL1L and SYVN1. In cancerous cells, GRP78 overexpression is associated with UPR activation, including upregulation of associated proteins such as PERK, ATF6, CHOP etc that further regulate JNK and NF-kB untimately leading to survival, tumor progression, angiogenesis, resistance to therapy and metastasis. GRP78 is a signaling receptor for activated alpha2-macroglobulin, plasminogen kringle 5, and microplasminogen, and plays role in viral entry of coxsackie B as well as dengue fever viruses. GRP78 is also involved in regulation of tissue factor procoagulant activity and functions as a receptor for angiogenic peptides via VEGFRs independent mechanism. Cell surface GRP78 is found associated with diverse proteins including VDAC, MHC-I, Cripto and DnaJ-like protein MTJ-1. Because GRP78 is present on the surface of cancer cells but not on normal cells in vivo, it provide an exciting opportunity for cancer targeting.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody [DyLight 405]

Certificate of Analysis

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Product Specific Notices for GRP78/HSPA5 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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