GRP78/HSPA5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16749

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human

Predicted:

Bovine (100%), Fish (95%), Rhesus Macaque (100%), Xenopus (99%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRP78/HSPA5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRP78/HSPA5. The exact sequence is proprietary.

Reactivity Notes

Japanese Medaka (95%), Xenopus laevis (99%).

Localization

Endoplasmic reticulum lumen; Melanosome

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRP78/HSPA5 Antibody - BSA Free

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749]

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749]

Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GRP78/HSPA5 Antibody

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] -

nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-25520231539516.jpg
GRP78/HSPA5 Antibody - BSA Free

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] -

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GRP78/HSPA5 Antibody - BSA Free

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] -

Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody (NBP2-16749) diluted at 1:250.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GRP78/HSPA5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] -

Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody (NBP2-16749) diluted at 1:500.
Blue: Hoechst 33342 staining.
GRP78/HSPA5 Antibody - BSA Free

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] -

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (NBP2-16749) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GRP78/HSPA5 Antibody - BSA Free

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] -

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GRP78/HSPA5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] -

Grp78 antibody detects Grp78 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
GRP78/HSPA5 Antibody - BSA Free

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (NBP2-16749) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GRP78/HSPA5 Antibody - BSA Free

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] -

Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 ug PC-12 whole cell extract
B. 30 ug Rat2 whole cell extract
7.5% SDS-PAGE
Grp78 antibody (NBP2-16749) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GRP78/HSPA5 Antibody - BSA Free

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] -

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for GRP78/HSPA5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

UniProt

P11021 (Human)

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRP78/HSPA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRP78/HSPA5 Antibody - BSA Free

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