GRPEL2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85099

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRPEL2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VRSLWAGRLRVQRLLAWSAAWESKGWPLPFSTATQRTAGEDCRSEDPPDELGPPLAERALRVKAVKLEKEVQDLTVRYQRAIADCENIRRRTQRCVEDAKIFGIQSFCKDLVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRPEL2 Antibody - BSA Free

Western Blot: GRPEL2 Antibody [NBP1-85099]

Western Blot: GRPEL2 Antibody [NBP1-85099]

Western Blot: GRPEL2 Antibody [NBP1-85099] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: GRPEL2 Antibody [NBP1-85099]

Immunocytochemistry/ Immunofluorescence: GRPEL2 Antibody [NBP1-85099]

Immunocytochemistry/Immunofluorescence: GRPEL2 Antibody [NBP1-85099] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Western Blot: GRPEL2 Antibody [NBP1-85099]

Western Blot: GRPEL2 Antibody [NBP1-85099]

Western Blot: GRPEL2 Antibody [NBP1-85099] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099] - Staining of human prostate shows strong cytoplasmic postivity in smooth muscle cells.
Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099] - Staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099]

Immunohistochemistry-Paraffin: GRPEL2 Antibody [NBP1-85099] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Applications for GRPEL2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRPEL2

Essential component of the PAM complex, a complex required for the translocation of transitpeptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems tocontrol the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity ofmt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms ofmt-HSP70

Alternate Names

FLJ23713, FLJ33918, GrpE-like 2, mitochondrial (E. coli), Mt-GrpE#2mitochondrial

Gene Symbol

GRPEL2

Additional GRPEL2 Products

Product Documents for GRPEL2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GRPEL2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRPEL2 Antibody - BSA Free

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Protocols

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