GSPT1 Antibody (HL1346) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25493
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Feline
Predicted:
Bovine (98%), Xenopus (91%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1346 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GSPT1 Antibody (HL1346) - Azide and BSA Free
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] -
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] -
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] -
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] -
Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] -
Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] -
Western Blot: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: GSPT1 Antibody (HL1346) [NBP3-25493] -
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for GSPT1 Antibody (HL1346) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GSPT1
Alternate Names
ERF3A, eRF3aFLJ38048, ETF3A, eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, FLJ39067, G1 to S phase transition 1,551G9.2, G1 to S phase transition protein 1 homolog, GST1
Gene Symbol
GSPT1
Additional GSPT1 Products
Product Documents for GSPT1 Antibody (HL1346) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GSPT1 Antibody (HL1346) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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