GSPT1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-16754

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GSPT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSPT1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-16754]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-16754]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-16754] - Human breast cancer, using GSPT1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GSPT1 Antibody - Azide and BSA Free

Western Blot: GSPT1 Antibody [NBP2-16754] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (NBP2-16754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GSPT1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-16754] -

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
GSPT1 stained by GSPT1 antibody (NBP2-16754) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GSPT1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSPT1 Antibody [NBP2-16754] -

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSPT1 stained by GSPT1 antibody (NBP2-16754) diluted at 1:500.
Blue: Fluoroshield with DAPI.
GSPT1 Antibody - Azide and BSA Free

Western Blot: GSPT1 Antibody [NBP2-16754] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (NBP2-16754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GSPT1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-16754] -

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GSPT1 stained by GSPT1 antibody (NBP2-16754) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for GSPT1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GSPT1

GSPT1 is involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates theactivity of ERF1. Involved in regulation of mammalian cell growth

Alternate Names

ERF3A, eRF3aFLJ38048, ETF3A, eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, FLJ39067, G1 to S phase transition 1,551G9.2, G1 to S phase transition protein 1 homolog, GST1

Gene Symbol

GSPT1

Additional GSPT1 Products

Product Documents for GSPT1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GSPT1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GSPT1 Antibody - Azide and BSA Free

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Protocols

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FAQs

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