GST Epitope Tag Antibody [HRP]

Novus Biologicals | Catalog # NB600-388

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative)

Cited:

Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all GST Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Rabbits were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.

Reactivity Notes

Rat reactivity in vitro transcription/translation systems reported in scientific literature (PMID:19412421). Human reactivity reported in in vitro transcription/translation systems scientific literature (PMID: 18614808). Bacteria reported in (PMID: 27470678).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GST Epitope Tag Antibody [HRP]

Western Blot: GST Epitope Tag Antibody [HRP] [NB600-388] - 1.0, 0.3, or 0.1 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Applications for GST Epitope Tag Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000-1:100000

Immunocytochemistry/ Immunofluorescence

1:200-1:500

Western Blot

1:1000 -1:30000
Application Notes
Immunohistochemistry Not applicable

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.05% Pro-Clean 400

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GST Epitope Tag

GST (glutathione S-transferase) is commonly engineered onto the N- or C- terminus of a protein of interest to create fusion proteins that can be purified, visualized and analyzed via the properties of the GST tag.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

Product Documents for GST Epitope Tag Antibody [HRP]

Certificate of Analysis

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Product Specific Notices for GST Epitope Tag Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GST Epitope Tag Antibody [HRP]

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Protocols

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FAQs

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