GST Epitope Tag Antibody - BSA Free

Novus Biologicals | Catalog # NB600-326

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Rat, Bacteria

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GST Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Rabbits were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.

Reactivity Notes

Rat reactivity in vitro transcription/translation systems reported in scientific literature (PMID:19412421). Human reactivity reported in in vitro transcription/translation systems scientific literature (PMID: 18614808). Bacteria reported in (PMID: 27470678).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GST Epitope Tag Antibody - BSA Free

Western Blot: GST Epitope Tag AntibodyBSA Free [NB600-326]

Western Blot: GST Epitope Tag AntibodyBSA Free [NB600-326]

Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).
Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).
Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag AntibodyBSA Free [NB600-326]

Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s).

Applications for GST Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:400

Immunoprecipitation

1-4 ug/mg lysate

Simple Western

1:2000

Western Blot

1:1000 - 1:30000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in hUbiquitn-GST and hISG15-GST lysates, hVAV1-GST, E. coli lysate and hPARK2-GST, conditioned media, separated by Size, antibody dilution of 1:2000, apparent MW was 39, 47, 43, 81 kDa. IHC-P reactivity reported in (PMID: 19412421).

Reviewed Applications

Read 2 reviews rated 5 using NB600-326 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GST Epitope Tag

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

Product Documents for GST Epitope Tag Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GST Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GST Epitope Tag Antibody - BSA Free

Customer Reviews for GST Epitope Tag Antibody - BSA Free (2)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: GST fustion protein
    Species: Other
    Verified Customer | Posted 01/07/2016
    GST fusion protein probed with GST antibody
    GST Epitope Tag Antibody - BSA Free NB600-326
  • Name: Darren Richard
    Application: Western Blot
    Sample Tested: GST pulldowns
    Species: Other
    Verified Customer | Posted 07/24/2015
    GST Western Blot
    GST Epitope Tag Antibody - BSA Free NB600-326

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Protocols

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FAQs

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