GSTA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33586

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GSTA1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTA1. The exact sequence is proprietary.

Reactivity Notes

Pig (85%), Sheep (82%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSTA1 Antibody - BSA Free

Immunohistochemistry-Paraffin: GSTA1 Antibody [NBP1-33586]

Immunohistochemistry-Paraffin: GSTA1 Antibody [NBP1-33586]

Immunohistochemistry-Paraffin: GSTA1 Antibody [NBP1-33586] - PC3 xenograft, using GSTA1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
GSTA1 Antibody

Immunocytochemistry/ Immunofluorescence: GSTA1 Antibody [NBP1-33586] -

Immunocytochemistry/ Immunofluorescence: GSTA1 Antibody [NBP1-33586] - GSTA1 antibody detects GSTA1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSTA1 stained by GSTA1 antibody (NBP1-33586) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10 um.
GSTA1 Antibody

Western Blot: GSTA1 Antibody [NBP1-33586] -

Western Blot: GSTA1 Antibody [NBP1-33586] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTA1 antibody (NBP1-33586) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GSTA1 Antibody - BSA Free

Western Blot: GSTA1 Antibody [NBP1-33586] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTA1 antibody (NBP1-33586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for GSTA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GSTA1

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq]

Long Name

Glutathione S-transferase A1

Alternate Names

EC 1.11.1.-, EC 2.5.1.18, EC 5.3.3.-, GST HA subunit 1, GST-epsilon, GSTA1-1

Entrez Gene IDs

2938 (Human)

Gene Symbol

GSTA1

UniProt

P08263 (Human)

Additional GSTA1 Products

Product Documents for GSTA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GSTA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for GSTA1 Antibody - BSA Free

Customer Reviews for GSTA1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review GSTA1 Antibody - BSA Free and earn rewards!

Have you used GSTA1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies