Guanylyl Cyclase alpha 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35266

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Guanylyl Cyclase alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Guanylyl Cyclase alpha 1 (NP_000847.2).

Sequence:
MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Guanylyl Cyclase alpha 1 Antibody - BSA Free

Guanylyl Cyclase alpha 1 Antibody

Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] -

Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] - Immunofluorescence analysis of L929 cells using Guanylyl Cyclase alpha 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Guanylyl Cyclase alpha 1 Antibody

Western Blot: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] -

Western Blot: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] - Western blot analysis of various lysates using Guanylyl Cyclase alpha 1 Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Applications for Guanylyl Cyclase alpha 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:1000 - 1:3000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Guanylyl Cyclase alpha 1

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit, such as alpha-1 (GUCY1A3), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM]

Long Name

Guanylate cyclase soluble subunit alpha-1

Alternate Names

EC 4.6.1.2, GCS-alpha-1, GCS-alpha-3, GUC1A3, GUCSA3, GUCY1A1, GUCY1A3

Gene Symbol

GUCY1A1

Additional Guanylyl Cyclase alpha 1 Products

Product Documents for Guanylyl Cyclase alpha 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Guanylyl Cyclase alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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