H2AFV Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04426

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all H2AFV Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFV (NP_036544.1). MAGGKAGKDSGKAKAKAVSRSQRAGLQFPVGRIHRHLKTRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for H2AFV Antibody - Azide and BSA Free

Western Blot: H2AFV AntibodyAzide and BSA Free [NBP3-04426]

Western Blot: H2AFV AntibodyAzide and BSA Free [NBP3-04426]

Western Blot: H2AFV Antibody [NBP3-04426] - Analysis of extracts of various cell lines, using H2AFV antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunohistochemistry-Paraffin: H2AFV Antibody - Azide and BSA Free [NBP3-04426]

Immunohistochemistry-Paraffin: H2AFV Antibody - Azide and BSA Free [NBP3-04426]

Immunohistochemistry-Paraffin: H2AFV Antibody [NBP3-04426] - Immunohistochemistry of paraffin-embedded Rat testis using H2AFV Rabbit pAb (NBP3-04426) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: H2AFV Antibody - Azide and BSA Free [NBP3-04426]

Immunocytochemistry/ Immunofluorescence: H2AFV Antibody - Azide and BSA Free [NBP3-04426]

Immunocytochemistry/Immunofluorescence: H2AFV Antibody [NBP3-04426] - Analysis of U-2 OS cells using H2AFV antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
H2AFV Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: H2AFV Antibody - Azide and BSA Free [H2AFV] -

Immunocytochemistry/ Immunofluorescence: H2AFV Antibody - Azide and BSA Free [H2AFV] - Immunofluorescence analysis of L929 cells using H2AFV Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
H2AFV Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: H2AFV Antibody - Azide and BSA Free [H2AFV] -

Immunocytochemistry/ Immunofluorescence: H2AFV Antibody - Azide and BSA Free [H2AFV] - Immunofluorescence analysis of C6 cells using H2AFV Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for H2AFV Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: H2AFV

H2AFV is 128 amino acids long, weighs approximately 13.5 kDa, and the gene codes for a variant component that is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. H2AFV has three shorter isoforms that are 114, 90, and 102 amino acids long, weighing 12, 9, and 11 kDa respectively. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, malaria, ataxia telangiectasia, intrahepatic cholangiocarcinoma, pharyngitis, and immunodeficiency. H2AFV has also been shown to have interactions with MORF4L1, MRGBP, PAX9, CAMSAP2, and ATG4C in pathways such as the systemic lupus erythematosus pathways.

Alternate Names

FLJ26479, H2A histone family, member V, H2A.F/Z, H2AV, histone H2A.F/Z, histone H2A.V, MGC10170, MGC10831, MGC1947, purine-rich binding element protein B

Gene Symbol

H2AZ2

Additional H2AFV Products

Product Documents for H2AFV Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for H2AFV Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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