HADH Antibody

Novus Biologicals | Catalog # NBP2-16776

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HADH Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HADH Antibody

Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776]

Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776]

Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776] - HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody dilution: 1:500.
Western Blot: HADH Antibody [NBP2-16776]

Western Blot: HADH Antibody [NBP2-16776]

Western Blot: HADH Antibody [NBP2-16776] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE
Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776]

Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776]

Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16776] - HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody dilution: 1:500.
Immunoprecipitation: HADH Antibody [NBP2-16776]

Immunoprecipitation: HADH Antibody [NBP2-16776]

Immunoprecipitation: HADH Antibody [NBP2-16776] - Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 ug of HADH antibody. Western blot analysis was performed using HADH antibody.
HADH Antibody

Western Blot: HADH Antibody [NBP2-16776] -

HADH antibody detects HADH protein by Western blot analysis.
A. 30 ug GL261 whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (NBP2-16776) dilution: 1:1000
HADH Antibody

Immunocytochemistry/ Immunofluorescence: HADH Antibody [NBP2-16776] -

HADH antibody detects HADH protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HADH protein stained by HADH antibody (NBP2-16776) diluted at 1:500.
Blue: Hoechst 33342 staining.
HADH Antibody

Western Blot: HADH Antibody [NBP2-16776] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HADH antibody (NBP2-16776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
HADH Antibody

Western Blot: HADH Antibody [NBP2-16776] -

HADH antibody detects HADH protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (NBP2-16776) dilution: 1:1000

Applications for HADH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HADH

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq]

Alternate Names

EC 1.1.1, EC 1.1.1.35, HADH1, HADHSChydroxyacyl-Coenzyme A dehydrogenase, HCDH, hydroxyacyl-CoA dehydrogenase, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, MGC8392, mitochondrial, MSCHAD, SCHADHHF4

Gene Symbol

HADH

Additional HADH Products

Product Documents for HADH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HADH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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