HAX-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31647

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HAX-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HAX-1. The exact sequence is proprietary.

Localization

Mitochondrion, Endoplasmic reticulum, Nucleus membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HAX-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HAX-1 Antibody [NBP1-31647]

Immunocytochemistry/ Immunofluorescence: HAX-1 Antibody [NBP1-31647]

Immunocytochemistry/Immunofluorescence: HAX-1 Antibody [NBP1-31647] - Analysis of methanol-fixed A431, using HAX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: HAX-1 Antibody [NBP1-31647]

Immunohistochemistry-Paraffin: HAX-1 Antibody [NBP1-31647]

Immunohistochemistry-Paraffin: HAX-1 Antibody [NBP1-31647] - Sample: Paraffin-embedded rat ovary. HAX-1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: HAX-1 Antibody [NBP1-31647]

Immunohistochemistry-Paraffin: HAX-1 Antibody [NBP1-31647]

Immunohistochemistry-Paraffin: HAX-1 Antibody [NBP1-31647] - NCIN87 Xenograft, using HAX1 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
HAX-1 Antibody

Immunocytochemistry/ Immunofluorescence: HAX-1 Antibody [NBP1-31647] -

Immunocytochemistry/ Immunofluorescence: HAX-1 Antibody [NBP1-31647] - HAX-1 antibody detects HAX-1 protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HAX-1 stained by HAX-1 antibody (NBP1-31647) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
HAX-1 Antibody

Western Blot: HAX-1 Antibody [NBP1-31647] -

Western Blot: HAX-1 Antibody [NBP1-31647] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HAX-1 antibody (NBP1-31647) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HAX-1 Antibody

Immunocytochemistry/ Immunofluorescence: HAX-1 Antibody [NBP1-31647] -

Immunocytochemistry/ Immunofluorescence: HAX-1 Antibody [NBP1-31647] - HAX-1 antibody detects HAX-1 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: HAX-1 stained by HAX-1 antibody (NBP1-31647) diluted at 1:500.

Applications for HAX-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HAX-1

HAX1 is known to associate with hematopoietic cell-specific Lyn substrate 1 (HS1), one of the substrates of receptor-coupled tyrosine kinases activated during clonal expansion and deletion in lymphoid cells. It also interacts with the product of the polycystic kidney disease 2 (PKD2) gene and with the F-actin-binding protein, cortactin. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.

Long Name

HCLS1 associated protein X-1

Alternate Names

HAX1, HCLSBP1, HS1BP1, SCN3

Gene Symbol

HAX1

Additional HAX-1 Products

Product Documents for HAX-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HAX-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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