HBG1/2 Antibody (JM84-10)

Novus Biologicals | Catalog # NBP2-66885

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM84-10 expressed in HEK293
View all HBG1/2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human HBG1/2 aa 2-45 / 147. (SwissProt: P69891 Human; SwissProt: P69892 Human)

Localization

Cytosol, hemoglobin complex.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HBG1/2 Antibody (JM84-10)

Western Blot: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Western Blot: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Western Blot: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of HBG1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: human placenta tissue lysateLane 2: human brain tissue lysate
Immunocytochemistry/ Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunocytochemistry/ Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of paraffin-embedded human tonsil tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunocytochemistry/ Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Hemoglobin gamma A Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunocytochemistry/ Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885]

Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of paraffin-embedded human spleen tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.

Applications for HBG1/2 Antibody (JM84-10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HBG1/2

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Alternate Names

abnormal hemoglobin, A-gamma globin, gamma A hemoglobin, gamma globin, Gamma-1-globin, gamma-2-globin, gamma-globin chain, G-gamma globin Paulinia, Hb F Agamma, hb F Ggamma, HBGA, HBGR, HBG-T1, HBG-T2, Hemoglobin gamma-1 chain, hemoglobin gamma-2 chain, Hemoglobin gamma-A chain, hemoglobin gamma-G chain, hemoglobin subunit gamma-1, hemoglobin subunit gamma-2, hemoglobin, gamma A, hemoglobin, gamma G, hemoglobin, gamma, regulator of, HSGGL1, methemoglobin, PRO2979, TNCY

Gene Symbol

HBG1

Additional HBG1/2 Products

Product Documents for HBG1/2 Antibody (JM84-10)

Certificate of Analysis

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Product Specific Notices for HBG1/2 Antibody (JM84-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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