HCN4 Antibody (SHG 1E5)

Novus Biologicals | Catalog # NB100-74439

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence

Cited:

Immunoblotting, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # SHG 1E5
View all HCN4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide sequence corresponding to residues S H G S L L L P P A S S P P P P Q V P Q R R G T P P L T P G R L T Q D L K L of HCN4.

Specificity

HCN4 (SHG 1E5)

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for HCN4 Antibody (SHG 1E5)

Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439]

Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439]

Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439] - Analysis of 25 ug of rat eye lysate onto an SDS polyacrylamide gel.
Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439]

Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439]

Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.
Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439]

Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439]

Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues.

Applications for HCN4 Antibody (SHG 1E5)

Application
Recommended Usage

Flow Cytometry

1:50

Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:1000

Immunohistochemistry-Paraffin

1:10 - 1:100

Western Blot

1:5000
Application Notes
WB: Detects an approx.132 KDa protein representing HCN4.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HCN4

HCN3 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. HCN3 has been detected in the brain, but with less expression than other HCN channels.

Long Name

Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4

Alternate Names

SSS2

Entrez Gene IDs

10021 (Human); 330953 (Mouse); 59266 (Rat)

Gene Symbol

HCN4

UniProt

Q9Y3Q4

Additional HCN4 Products

Product Documents for HCN4 Antibody (SHG 1E5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HCN4 Antibody (SHG 1E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HCN4 Antibody (SHG 1E5)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HCN4 Antibody (SHG 1E5)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I need an anti human HCN4 antibody for sorting. I was wondering if your product HCN4 antibody (NB110-74735) can react with the extracellular portions of HCN4 and is applicable for FACS?

    A: NB110-74735 was raised against a synthetic peptide corresponding to a region that covered both cytoplasmic and extracellular regions of the HCN4 protein. We don't routinely map our antibodies, and we don't know where specifically this antibody binds its target. NB100-74439 may be a better option for you, as the immunogen sequence is provided on our website, but we have not validated it for Flow/FACS.

  • Q: I was looking for HCN4 antiboby that attaches to the extra cellular domain of the channel tested on mouse and human for immunio stoaining..

    A: The immunogen for our monoclonal antibody to HCN4, is actually the rat protein. The sequence comes from amino acids 1046 - 1083 of the 1198 amino acid protein, but unfortunately, it is from a cytoplasmic domain. This is the only HCN4 Ab that we have tested for IHC and that has been validated for use with mouse and human.

  • Q: I need an anti human HCN4 antibody for sorting. I was wondering if your product HCN4 antibody (NB110-74735) can react with the extracellular portions of HCN4 and is applicable for FACS?

    A: NB110-74735 was raised against a synthetic peptide corresponding to a region that covered both cytoplasmic and extracellular regions of the HCN4 protein. We don't routinely map our antibodies, and we don't know where specifically this antibody binds its target. NB100-74439 may be a better option for you, as the immunogen sequence is provided on our website, but we have not validated it for Flow/FACS.

  • Q: I was looking for HCN4 antiboby that attaches to the extra cellular domain of the channel tested on mouse and human for immunio stoaining..

    A: The immunogen for our monoclonal antibody to HCN4, is actually the rat protein. The sequence comes from amino acids 1046 - 1083 of the 1198 amino acid protein, but unfortunately, it is from a cytoplasmic domain. This is the only HCN4 Ab that we have tested for IHC and that has been validated for use with mouse and human.

Showing  1 - 2 of 2 FAQs Showing All
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