HDAC1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56340

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 1/HDAC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 1-5, 433-448 and 467-482 of human HDAC1 (Genbank Accession no. Q13547).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25648995). Rat reactivity reported in scientific literature (PMID: 26551542)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC1 Antibody - BSA Free

Knockout Validated: HDAC1 Antibody [NB100-56340]

Western Blot: HDAC1 Antibody [NB100-56340]

Western Blot: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: HDAC1 Antibody [NB100-56340]

Western Blot: HDAC1 Antibody [NB100-56340]

Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Simple Western: HDAC1 Antibody [NB100-56340]

Simple Western: HDAC1 Antibody [NB100-56340]

Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for HDAC1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500. Use reported in multiple pieces of scientific literature

Immunohistochemistry-Frozen

1:10-1:500. Use reported in scientific literature (PMID 26551542)

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (PMID 25648995)

Simple Western

1:200

Western Blot

2 ug/ml
Application Notes

In 293, a 60 kDa band is observed. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 68 kDa

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-56340 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 1/HDAC1

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Entrez Gene IDs

3065 (Human)

Gene Symbol

HDAC1

UniProt

Q01101 (Human)

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HDAC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Notch Pathway

Citations for HDAC1 Antibody - BSA Free

Customer Reviews for HDAC1 Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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  • HDAC1 Antibody
    Name: Aileen Keating
    Application: Western Blot
    Sample Tested: Ovary tissue
    Species: Pig
    Verified Customer | Posted 11/10/2016
    HDAC on porcine whole ovarian homogenate (50 ug protein)
    -Blocked in 5% BSA.2% PBST for 4 hours -Primary was 1:250 in 5% BSA.2% PBST overnight at 4C -Secondary was goat x rabbit 1:1000 in 5% BSA.2% PBST 1 hour
    HDAC1 Antibody - BSA Free NB100-56340
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23327920
    Species: Mouse
    Verified Customer | Posted 12/12/2014

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Protocols

View specific protocols for HDAC1 Antibody - BSA Free (NB100-56340):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in 2% Non-fat milk in TBST and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructi

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