HDAC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31294

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (98%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), SDS-Page

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 1/HDAC1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (80%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC1 Antibody - BSA Free

Knockout Validated: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.
Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294]

Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294]

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294]

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
HDAC1 Antibody

Western Blot: HDAC1 Antibody [NBP1-31294] -

Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HDAC1 Antibody

Western Blot: HDAC1 Antibody [NBP1-31294] -

Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HDAC1 Antibody

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] -

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC1 stained by HDAC1 antibody (NBP1-31294) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Scale bar= 10um.
HDAC1 Antibody

Immunohistochemistry: HDAC1 Antibody [NBP1-31294] -

Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (NBP1-31294) at 1:300 dilution.
HDAC1 Antibody

Western Blot: HDAC1 Antibody [NBP1-31294] -

Western Blot: HDAC1 Antibody [NBP1-31294] - Sample (30 ug of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
NBP1-31294 diluted at 1:1000
HDAC1 Antibody

Immunohistochemistry: HDAC1 Antibody [NBP1-31294] -

Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
HDAC1 antibody (NBP1-31294) dilution: 1:100.
HDAC1 Antibody

Western Blot: HDAC1 Antibody [NBP1-31294] -

Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HDAC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 1/HDAC1

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Gene Symbol

HDAC1

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HDAC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Epigenetics
Notch Pathway

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Protocols

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