HDAC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83350

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 1/HDAC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-83350]

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-83350]

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-83350] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
HDAC1 Antibody - BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Staining of human small intestine shows moderate nuclear positivity in glandular cells.
HDAC1 Antibody - BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
HDAC1 Antibody - BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Staining of human tonsil shows moderate nuclear positivity in germinal center cells.
HDAC1 Antibody - BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
HDAC1 Antibody - BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Immunohistochemistry-Paraffin: HDAC1 Antibody - BSA Free [NBP1-83350]

Analysis in human tonsil and skeletal muscle tissues using NBP1-83350 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.
HDAC1 Antibody - BSA Free Western Blot: HDAC1 Antibody - BSA Free [NBP1-83350]

Western Blot: HDAC1 Antibody - BSA Free [NBP1-83350]

Analysis in RT-4 cells) transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for HDAC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 1/HDAC1

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Entrez Gene IDs

3065 (Human)

Gene Symbol

HDAC1

UniProt

Q13547 (Human)

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HDAC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Notch Pathway

Citations for HDAC1 Antibody - BSA Free

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Protocols

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FAQs

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