HDAC3 Antibody (HL1749) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25497

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Drosophila

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1749 expressed in HEK293

Format

Azide and BSA Free
View all Histone Deacetylase 3/HDAC3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC3 Antibody (HL1749) - Azide and BSA Free

HDAC3 Antibody (HL1749) - Azide and BSA Free

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] -

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HDAC3 Antibody (HL1749) - Azide and BSA Free

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] -

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HDAC3 Antibody (HL1749) - Azide and BSA Free

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] -

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
HDAC3 Antibody (HL1749) - Azide and BSA Free

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] -

Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HDAC3 Antibody (HL1749) - Azide and BSA Free

Immunohistochemistry-Paraffin: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] -

Immunohistochemistry-Paraffin: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. HDAC3 stained by HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HDAC3 Antibody (HL1749) - Azide and BSA Free

Immunohistochemistry-Paraffin: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] -

Immunohistochemistry-Paraffin: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HDAC3 stained by HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for HDAC3 Antibody (HL1749) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Gene Symbol

HDAC3

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody (HL1749) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HDAC3 Antibody (HL1749) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for HDAC3 Antibody (HL1749) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review HDAC3 Antibody (HL1749) - Azide and BSA Free and earn rewards!

Have you used HDAC3 Antibody (HL1749) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies