HDAC5 Antibody (CL8121) - BSA Free

Novus Biologicals | Catalog # NBP2-88913

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL8121

Format

BSA Free
View all Histone Deacetylase 5/HDAC5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q9UQL6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HDAC5 Antibody (CL8121) - BSA Free

Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunocytochemistry/Immunofluorescence: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of RH-30 cells using the Anti-HDAC5 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human stomach cancer shows moderate to strong cytoplasmic and nuclear positivity.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913]

Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Analysis in human cerebral cortex and liver tissues using NBP2-88913 antibody. Corresponding HDAC5 RNA-seq data are presented for the same tissues.

Applications for HDAC5 Antibody (CL8121) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 5/HDAC5

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF(p300/CBP- associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Long Name

Histone Deacetylase 5

Alternate Names

FLJ90614, KIAA0600, NY-CO-9

Gene Symbol

HDAC5

Additional Histone Deacetylase 5/HDAC5 Products

Product Documents for HDAC5 Antibody (CL8121) - BSA Free

Certificate of Analysis

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Product Specific Notices for HDAC5 Antibody (CL8121) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Epigenetics

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Protocols

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