HDAC9 Antibody (1F2) - BSA Free

Novus Biologicals | Catalog # NBP3-26235

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1F2 expressed in HEK293

Format

BSA Free
View all HDAC9 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human HDAC9 [UniProt Q9UKV0]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC9 Antibody (1F2) - BSA Free

HDAC9 Antibody (1F2)

Immunohistochemistry: HDAC9 Antibody (1F2) [NBP3-26235] -

Immunohistochemistry: HDAC9 Antibody (1F2) [NBP3-26235] - Image of HDAC9 Antibody (1F2) diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HDAC9 Antibody (1F2)

Immunohistochemistry: HDAC9 Antibody (1F2) [NBP3-26235] -

Immunohistochemistry: HDAC9 Antibody (1F2) [NBP3-26235] - Image of HDAC9 Antibody (1F2) diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HDAC9 Antibody (1F2)

Western Blot: HDAC9 Antibody (1F2) [NBP3-26235] -

Western Blot: HDAC9 Antibody (1F2) [NBP3-26235] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate.
All lanes: HDAC9 Antibody at 1.54ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 112, 102, 66, 98, 118, 113, 61, 63, 58 KDa
Observed band size: 160 KDa
HDAC9 Antibody (1F2)

Immunocytochemistry/Immunofluorescence: HDAC9 Antibody (1F2) [NBP3-26235] -

Immunocytochemistry/Immunofluorescence: HDAC9 Antibody (1F2) [NBP3-26235] - Staining of Hela cells with HDAC9 Antibody (1F2) at 1:51, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for HDAC9 Antibody (1F2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HDAC9

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Long Name

Histone deacetylase 9

Alternate Names

HD7b, HD9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR

Gene Symbol

HDAC9

Additional HDAC9 Products

Product Documents for HDAC9 Antibody (1F2) - BSA Free

Certificate of Analysis

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Product Specific Notices for HDAC9 Antibody (1F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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