HE4/WFDC2 Antibody (JF62-09)

Novus Biologicals | Catalog # NBP2-66883

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF62-09 expressed in HEK293
View all HE4/WFDC2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human HE4/WFDC2 aa 78-114 / 124. (SwissProt: Q14508 Human; SwissProt: Q9DAU7 Mouse)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HE4/WFDC2 Antibody (JF62-09)

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.
Flow Cytometry: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883]

Flow Cytometry: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883]

Flow Cytometry: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Flow cytometric analysis of SW480 cells with HE4/WFDC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
HE4/WFDC2 Antibody (JF62-09)

Western Blot: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] -

Western Blot: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - analysis of HE4 on different lysates with Rabbit anti-HE4 antibody (ET1702-61) at 1/2,000 dilution.Lane 1: SW480 cell lysate (15 ug/Lane)Lane 2: HepG2 cell lysate (15 ug/Lane)Lane 3: OVCAR-3 cell lysate (15 ug/Lane)Lane 4: Mouse lung tissue lysate (20 ug/Lane)Lane 5: Mouse kidney tissue lysate (20 ug/Lane)Predicted band size: 13 kDaObserved band size: 20 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HE4/WFDC2 Antibody (JF62-09)

Immunocytochemistry/Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] -

Immunocytochemistry/Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - analysis of HepG2 cells labeling HE4 with Rabbit anti-HE4 antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HE4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4c. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Applications for HE4/WFDC2 Antibody (JF62-09)

Application
Recommended Usage

Flow Cytometry

1:1,000

Immunocytochemistry/ Immunofluorescence

IF-Cell 1:50-1:200, IF-Tissue 1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

Assay dependent concentration

Western Blot

1:2,000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HE4/WFDC2

The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Gene Symbol

WFDC2

Additional HE4/WFDC2 Products

Product Documents for HE4/WFDC2 Antibody (JF62-09)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HE4/WFDC2 Antibody (JF62-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HE4/WFDC2 Antibody (JF62-09)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HE4/WFDC2 Antibody (JF62-09)

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  • Q: I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?

    A:

    At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

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