Hemogen Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-84907
![Western Blot: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Western-Blot-NBP1-84907-img0010.jpg "Western Blot: Hemogen Antibody [NBP1-84907]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hemogen Antibody - BSA Free
Western Blot: Hemogen Antibody [NBP1-84907]
Western Blot: Hemogen Antibody [NBP1-84907] - Analysis in control (vector only transfected HEK293T lysate) and HEMGN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Immunohistochemistry-Paraffin-NBP1-84907-img0019.jpg "Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]")
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Immunohistochemistry-Paraffin-NBP1-84907-img0007.jpg "Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]")
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human bone marrow shows high expression.![Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Immunohistochemistry-Paraffin-NBP1-84907-img0015.jpg "Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]")
![Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Immunohistochemistry-Paraffin-NBP1-84907-img0016.jpg "Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]")
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human cerebral cortex shows no positivity in neurons as expected.![Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Immunohistochemistry-Paraffin-NBP1-84907-img0017.jpg "Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]")
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human colon shows no positivity in glandular cells as expected.![Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Immunohistochemistry-Paraffin-NBP1-84907-img0018.jpg "Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]")
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human kidney shows no positivity in cells in tubules.![Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]](https://resources.rndsystems.com/images/products/Hemogen-Antibody-Immunohistochemistry-Paraffin-NBP1-84907-img0020.jpg "Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]")
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907]
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human bone marrow, colon, kidney and testis using Anti-HEMGN antibody (A) NBP1-84907 shows similar protein distribution across tissues to independent antibody NBP1-84909 (B).Applications for Hemogen Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Hemogen
Alternate Names
EDAGEDAG-1, Erythroid differentiation-associated gene protein, hemogen, Hemopoietic gene protein, NDR, Negative differentiation regulator protein
Gene Symbol
HEMGN
Additional Hemogen Products
Product Documents for Hemogen Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Hemogen Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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