Hemoglobin A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05569

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Hemoglobin A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human Hemoglobin A2 (NP_000508.1). MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hemoglobin A2 Antibody - BSA Free

Western Blot: Hemoglobin A2 AntibodyBSA Free [NBP3-05569]

Western Blot: Hemoglobin A2 AntibodyBSA Free [NBP3-05569]

Western Blot: Hemoglobin A2 Antibody [NBP3-05569] - Western blot analysis of extracts of mouse liver, using Hemoglobin A2 antibody (NBP3-05569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Hemoglobin A2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Hemoglobin A2 Antibody - BSA Free [NBP3-05569] -

Immunocytochemistry/ Immunofluorescence: Hemoglobin A2 Antibody - BSA Free [NBP3-05569] - Immunofluorescence analysis of Mouse placenta using Hemoglobin A2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Hemoglobin A2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Hemoglobin A2 Antibody - BSA Free [NBP3-05569] -

Immunocytochemistry/ Immunofluorescence: Hemoglobin A2 Antibody - BSA Free [NBP3-05569] - Immunofluorescence analysis of Rat placenta using Hemoglobin A2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Hemoglobin A2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Hemoglobin A2 Antibody - BSA Free [NBP3-05569] -

Immunocytochemistry/ Immunofluorescence: Hemoglobin A2 Antibody - BSA Free [NBP3-05569] - Immunofluorescence analysis of Human placenta using Hemoglobin A2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Hemoglobin A2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hemoglobin A2

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta -pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) codingsequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but theydiffer significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA,which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains toconstitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemiasresult from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alphathalassemias have also been reported. (provided by RefSeq)

Alternate Names

alpha globin, alpha-2 globin, alpha-globin, Hemoglobin alpha chain, hemoglobin subunit alpha, hemoglobin, alpha 2

Gene Symbol

HBA2

Additional Hemoglobin A2 Products

Product Documents for Hemoglobin A2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Hemoglobin A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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