HES-1 Antibody (OTI4H1)
Novus Biologicals | Catalog # NBP1-47791
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)
Cited:
Western Blot, Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4H1
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Product Specifications
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens hairy and enhancer of split 1, (Drosophila) (HES1).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HES-1 Antibody (OTI4H1)
![Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Western-Blot-NBP1-47791-img0018.jpg "Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791]
Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).![Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0010.jpg "Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791]
Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0015.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.![Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0008.jpg "Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791]
Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0011.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded breast using anti-Hes1 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0012.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded Carcinoma of liver using anti-Hes1 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0013.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded endometrium using anti-Hes1 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]](https://resources.rndsystems.com/images/products/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0014.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]")
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791]
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.Applications for HES-1 Antibody (OTI4H1)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Reactivity reported in (PMID: 29048612)
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HES-1
Long Name
Hairy And Enhancer of Split 1
Alternate Names
HES1, HL, HRY
Entrez Gene IDs
3280 (Human)
Gene Symbol
HES1
UniProt
Additional HES-1 Products
Product Documents for HES-1 Antibody (OTI4H1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HES-1 Antibody (OTI4H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HES-1 Antibody (OTI4H1)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for HES-1 Antibody (OTI4H1)
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Q: Can you elaborate in detail regarding the specificity mentioned for NBP1-47791?
A: This antibody has been created to detect the human HES1 protein (UniProt Q14469). The immunogen information given states that the immunogen corresponds to amino acids 1-280 of human HES1, which according to the UniProt entry I've just linked to is the whole protein. I think the mention of Drosophila in the specificity information is referring to the derivation of the name of this protein. Wikipedia says, 'Transcription factor HES1 (hairy and enhancer of split-1) is a protein that is encoded by the Hes1 gene, and is the mammalian homolog of the hairy gene in Drosophila'.
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Associated Pathways
Notch Signaling Pathways
