HES5 Antibody (4S4U7)

Name: HES5 Antibody (4S4U7)
Brand: Novus Biologicals
SKU: NBP3-16880
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16880

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4S4U7 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 12-142 of human HES5 (Q5TA89). SPKEKNRLRKPVVEKMRRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLKHSKAFVAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQRPPAAPAAPAKEPKAPGAAPPP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HES5 Antibody (4S4U7)

Western Blot: HES5 Antibody (4S4U7) [NBP3-16880]

Western Blot: HES5 Antibody (4S4U7) [NBP3-16880] - Analysis of extracts of various cell lines, using HES5 Rabbit mAb (NBP3-16880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry: HES5 Antibody (4S4U7) [NBP3-16880] -

Immunohistochemistry: HES5 Antibody (4S4U7) [NBP3-16880] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using HES5 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for HES5 Antibody (4S4U7)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HES-5

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.

Long Name

Hairy And Enhancer of Split 5

Alternate Names

HES5

Gene Symbol

HES5

Additional HES-5 Products

Product Documents for HES5 Antibody (4S4U7)

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Certificate of Analysis

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Product Specific Notices for HES5 Antibody (4S4U7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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