Hexokinase 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31600

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Zebrafish

Cited:

Bovine

Predicted:

Mouse (94%), Porcine (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Hexokinase 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hexokinase 1. The exact sequence is proprietary.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 31261667). Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%), Xenopus laevis (86%).

Localization

Mitochondrion outer membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hexokinase 1 Antibody - BSA Free

Western Blot: Hexokinase 1 Antibody [NBP1-31600]

Western Blot: Hexokinase 1 Antibody [NBP1-31600]

Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Sample (30 ug of whole cell lysate)C: zebrafish muscle7.5% SDS PAGEdiluted at 1:1000
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody [NBP1-31600]

Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody [NBP1-31600]

Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody [NBP1-31600] - Analysis of methanol-fixed HeLa, using HXK I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry Whole-Mount: Hexokinase 1 Antibody [NBP1-31600]

Immunohistochemistry Whole-Mount: Hexokinase 1 Antibody [NBP1-31600]

Immunohistochemistry Whole-Mount: Hexokinase 1 Antibody [NBP1-31600] - Hexokinase 1 antibody detects Hexokinase 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo.Hexokinase 1 antibody dilution: 1:100.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Human breast cancer, using HXK Iantibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Analysis of paraffin-embedded zebrafish tissue, using Hexokinase 1 antibody at 1:300 dilution.
Hexokinase 1 Antibody

Western Blot: Hexokinase 1 Antibody [NBP1-31600] -

Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase 1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Hexokinase 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC-WM-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq]

Alternate Names

HK1, HKI

Gene Symbol

HK1

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Hexokinase 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Hexokinase 1 Antibody - BSA Free

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