HFE Antibody (1G12) - Azide and BSA Free

Novus Biologicals | Catalog # H00003077-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G12

Format

Azide and BSA Free
View all HFE Primary Antibodies »

Product Specifications

Immunogen

HFE (NP_000401, 115 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQ

Specificity

HFE - hemochromatosis

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HFE Antibody (1G12) - Azide and BSA Free

Western Blot: HFE Antibody (1G12) [H00003077-M01]

Western Blot: HFE Antibody (1G12) [H00003077-M01]

Western Blot: HFE Antibody (1G12) [H00003077-M01] - Analysis of HFE expression in transfected 293T cell line by HFE monoclonal antibody (M01), clone 1G12.Lane 1: HFE transfected lysate(40.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: HFE Antibody (1G12) [H00003077-M01]

Immunocytochemistry/ Immunofluorescence: HFE Antibody (1G12) [H00003077-M01]

Immunocytochemistry/Immunofluorescence: HFE Antibody (1G12) [H00003077-M01] - Analysis of monoclonal antibody to HFE on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: HFE Antibody (1G12) [H00003077-M01]

Western Blot: HFE Antibody (1G12) [H00003077-M01]

Western Blot: HFE Antibody (1G12) [H00003077-M01] - HFE monoclonal antibody (M01), clone 1G12. Analysis of HFE expression in A-431.
ELISA: HFE Antibody (1G12) [H00003077-M01]

ELISA: HFE Antibody (1G12) [H00003077-M01]

ELISA: HFE Antibody (1G12) [H00003077-M01] - Detection limit for recombinant GST tagged HFE is approximately 0.03ng/ml as a capture antibody.

Applications for HFE Antibody (1G12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reviewed Applications

Read 2 reviews rated 4.5 using H00003077-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HFE

The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.

Long Name

Homeostatic Iron Regulator

Alternate Names

HFE1, HLA-H, MVCD7, TFQTL2

Entrez Gene IDs

3077 (Human)

Gene Symbol

HFE

OMIM

176200 (Human)

UniProt

NP_000401 (Human)

Additional HFE Products

Product Documents for HFE Antibody (1G12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HFE Antibody (1G12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HFE Antibody (1G12) - Azide and BSA Free

Customer Reviews for HFE Antibody (1G12) - Azide and BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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  • HFE Antibody (1G12)
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 06/27/2017
    HFE Antibody (1G12) - Azide and BSA Free H00003077-M01
  • HFE Antibody (1G12)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 06/21/2017
    HFE Antibody (1G12) - Azide and BSA Free H00003077-M01

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Protocols

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FAQs

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