HIPK3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-24521
![Western Blot: HIPK3 AntibodyBSA Free [NBP2-24521]](https://resources.rndsystems.com/images/products/HIPK3-Antibody-Western-Blot-NBP2-24521-img0003.jpg "Western Blot: HIPK3 AntibodyBSA Free [NBP2-24521]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Primate
Predicted:
Chimpanzee (100%), Monkey (94%), Orangutan (94%), Rhesus Macaque (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A portion of amino acids 850-900 of human HIPK3 was used as the immunogen.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Equine (83%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HIPK3 Antibody - BSA Free
Western Blot: HIPK3 AntibodyBSA Free [NBP2-24521]
Western Blot: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunocytochemistry/ Immunofluorescence: HIPK3 Antibody - BSA Free [NBP2-24521]](https://resources.rndsystems.com/images/products/HIPK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24521-img0006.jpg "Immunocytochemistry/ Immunofluorescence: HIPK3 Antibody - BSA Free [NBP2-24521]")
Immunocytochemistry/ Immunofluorescence: HIPK3 Antibody - BSA Free [NBP2-24521]
Immunocytochemistry/Immunofluorescence: HIPK3 Antibody [NBP2-24521] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HIPK3 [NBP2-24521] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]](https://resources.rndsystems.com/images/products/HIPK3-Antibody-Immunohistochemistry-Paraffin-NBP2-24521-img0005.jpg "Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]")
Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]
Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of human testis using this antibody at 10 ug/ml.![Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]](https://resources.rndsystems.com/images/products/HIPK3-Antibody-Immunohistochemistry-Paraffin-NBP2-24521-img0001.jpg "Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]")
Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]
Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.Applications for HIPK3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIPK3
Alternate Names
Androgen receptor-interacting nuclear protein kinase, DYRK6Fas-interacting serine/threonine-protein kinase, EC 2.7.11.1, FIST, FIST3ANPK, homeodomain interacting protein kinase 3, homeodomain-interacting protein kinase 3, PKYHomolog of protein kinase YAK1, YAK1
Gene Symbol
HIPK3
UniProt
Additional HIPK3 Products
Product Documents for HIPK3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIPK3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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