His Tag Antibody (359) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43536

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 359

Format

Azide and BSA Free
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to His Tag

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for His Tag Antibody (359) - Azide and BSA Free

Simple Western: His Tag Antibody (359) [NBP2-43536]

Simple Western: His Tag Antibody (359) [NBP2-43536]

Simple Western: His Tag Antibody (359) [NBP2-43536] - Human purified protein. Simple Western image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: His Tag Antibody (359) [NBP2-43536]

Immunocytochemistry/ Immunofluorescence: His Tag Antibody (359) [NBP2-43536]

Immunocytochemistry/Immunofluorescence: His Tag Antibody (359) [NBP2-43536] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 6X His tag stained by 6X His tag antibody [359] diluted at 1:1000.Blue: Fluoroshield with DAPI.
Western Blot: His Tag Antibody (359) [NBP2-43536]

Western Blot: His Tag Antibody (359) [NBP2-43536]

Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis. A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins. B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins. 15 % SDS-PAGE. 6x His antibody dilution: 1:5000.
Western Blot: His Tag Antibody (359) [NBP2-43536]

Western Blot: His Tag Antibody (359) [NBP2-43536]

Western Blot: His Tag Antibody (359) [NBP2-43536] - Analysis of cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 ug of 6X His tag antibody [359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [359] diluted at 1:1000.
ELISA: His Tag Antibody (359) [NBP2-43536]

ELISA: His Tag Antibody (359) [NBP2-43536]

ELISA: His Tag Antibody (359) [NBP2-43536] - 6X His tag antibody [359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 ug/mL 6X His tag antibody [359]. Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.
His Tag Antibody (359)

Western Blot: His Tag Antibody (359) [NBP2-43536] -

Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12 % SDS-PAGE
6x His antibody (NBP2-43536) dilution: 1:5000
His Tag Antibody (359) - Azide and BSA Free

Western Blot: His Tag Antibody (359) [NBP2-43536] -

6x His antibody detects 6x His protein by Western blot analysis.
A. 2 ug GST-recombinant protein
B. 2 ug His-recombinant protein
C. 1 ug His-recombinant protein
D. 0.5 ug His-recombinant protein E. 0.1 ug His-recombinant protein
E. 0.1 ug His-recombinant protein E. 0.1 ug His-recombinant protein
12 % SDS-PAGE
6x His antibody (NBP2-43536) dilution: 1:5000
His Tag Antibody (359) - Azide and BSA Free

Western Blot: His Tag Antibody (359) [NBP2-43536] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (NBP2-43536) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for His Tag Antibody (359) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:10000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:2000

Immunoprecipitation

1:100 - 1:500

Simple Western

Validated by a verified customer review

Western Blot

1:1000 - 1:10000

Reviewed Applications

Read 1 review rated 5 using NBP2-43536 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (359) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for His Tag Antibody (359) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for His Tag Antibody (359) - Azide and BSA Free (1)

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  • His Tag Antibody (359)
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 12/04/2018
    His Tag Antibody (359) - Azide and BSA Free NBP2-43536

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