His Tag Antibody (AD1.1.10) [Biotin]

Name: His Tag Antibody (AD1.1.10) [Biotin]
Brand: Novus Biologicals
SKU: NB100-63172
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-63172

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Epitope Tag

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # AD1.1.10

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Product Specifications

Immunogen

This His Tag Antibody (AD1.1.10) was developed against PAX6 transcription factor linked to histidine tag.

Reactivity Notes

6x His Tag specific

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for His Tag Antibody (AD1.1.10) [Biotin]

Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [Biotin] [NB100-63172] - Western blot of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.

Applications for His Tag Antibody (AD1.1.10) [Biotin]

Application

Recommended Usage

ELISA

1:500-1:1000

Western Blot

1:1000

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

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Product Documents for His Tag Antibody (AD1.1.10) [Biotin]

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Certificate of Analysis

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Product Specific Notices for His Tag Antibody (AD1.1.10) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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