His Tag Biotinylated Antibody

(4 citations)
(4 Reviews)
  
  • Specificity
    Detects proteins containing accessible consecutive histidine regions. This antibody detects His tags localized at the amino- or carboxyl-terminus.
  • Source
    Monoclonal Mouse IgG1 Clone # AD1.1.10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    His-tagged peptide
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of His Tag by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
  • Long Name:
    Histidine Tag
  • Alternate Names:
    6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Antibodies Targeting Closely Adjacent or Minimally Overlapping Epitopes Can Displace One Another
    Authors: YN Abdiche, AY Yeung, I Ni, D Stone, A Miles, W Morishige, A Rossi, P Strop
    PLoS ONE, 2017;12(1):e0169535.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  2. High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity.
    Authors: Abdiche, Yasmina, Miles, Adam, Eckman, Josh, Foletti, Davide, Van Blarcom, Thomas J, Yeung, Yik Andy, Pons, Jaume, Rajpal, Arvind
    PLoS ONE, 2014;9(3):e92451.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  3. Designed ankyrin repeat proteins: a new approach to mimic complex antigens for diagnostic purposes?
    Authors: Hausammann, Stefanie, Vogel, Monique, Kremer Hovinga, Johanna, Lacroix-Desmazes, Sebastie, Stadler, Beda M, Horn, Michael
    PLoS ONE, 2013;8(4):e60688.
    Species: Human
    Sample Type:
    Application: Binding Assay
  4. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
    Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
    Int. J. Cancer, 2006;119(2):349-58.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Biotinylated Antibody

Ctrl IC002B 2
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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Average Rating: 3.8 (Based on 4 reviews)

Have you used His Tag Biotinylated Antibody?

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Read what people are saying who have used His Tag Biotinylated Antibody.

We have 4 reviews tested in 3 species: E. coli, Mouse, Rabbit.
We have 4 reviews tested in 3 applications: Affinity Purification, Western Blot, biosensor capture assay..

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Very Good
(3)
Average
(1)
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Affinity Purification
(1)
WB
(2)
biosensor capture assay.
(1)
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E. coli
(1)
Mouse
(2)
Rabbit
(1)
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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Affinity Purification Rabbit Anonymous 12/01/2017
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Summary

ApplicationAffinity Purification
Sample Testedantigen specific B cells
SpeciesRabbit
  Average
 WB Mouse Anonymous 05/16/2016
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Summary

ApplicationWestern Blot
Sample TestedSerum
SpeciesMouse
Western Blot His Tag Biotinylated Antibody BAM050
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Very Good
 WB E. coli Anonymous 02/23/2016
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Image Details
Western Blot His Tag Biotinylated Antibody BAM050
Western Blot: His Tag Biotinylated Antibody [BAM050]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesE. coli

Other Experimental Details

Other Experimental DetailsAntibody was used to probe cell lysates from E. coli to identify protein of interest which carries a C-terminal his-tag. The yield of our protein is very low in the soluble fraction, so we used this mAb to compare the soluble fraction to the inclusion body prep to see if there was a signal in the inclusion body prep indicating that our protein may not be soluble.
  Very Good
 biosensor capture assay. Mouse Anonymous 02/22/2016
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Summary

Applicationbiosensor capture assay.
Sample TestedMonoclonal Mouse IgG1 Clone # AD1.1.10
SpeciesMouse

Other Experimental Details

Other Experimental DetailsThis antibody was used to capture his tagged proteins in various biosensor assays. This antibody works fine for general purposes. In some platforms (i.e., Octet), it is not as robust as a capture reagent, but it is still acceptable.

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