His Tag Biotinylated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of His Tag by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
Citations for His Tag Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Antibodies Targeting Closely Adjacent or Minimally Overlapping Epitopes Can Displace One Another
Authors: YN Abdiche, AY Yeung, I Ni, D Stone, A Miles, W Morishige, A Rossi, P Strop
PLoS ONE, 2017;12(1):e0169535.
Sample Types: Recombinant Protein
Applications: ELISA Development
High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity.
Authors: Abdiche, Yasmina, Miles, Adam, Eckman, Josh, Foletti, Davide, Van Blarcom, Thomas J, Yeung, Yik Andy, Pons, Jaume, Rajpal, Arvind
PLoS ONE, 2014;9(3):e92451.
Sample Types: Protein
Designed ankyrin repeat proteins: a new approach to mimic complex antigens for diagnostic purposes?
Authors: Hausammann, Stefanie, Vogel, Monique, Kremer Hovinga, Johanna, Lacroix-Desmazes, Sebastie, Stadler, Beda M, Horn, Michael
PLoS ONE, 2013;8(4):e60688.
Applications: Binding Assay
Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
Int. J. Cancer, 2006;119(2):349-58.
Sample Types: Whole Tissue
Applications: IHC Frozen
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Reviews for His Tag Biotinylated Antibody
Average Rating: 4 (Based on 5 Reviews)
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Antibody was used to probe cell lysates from E. coli to identify protein of interest which carries a C-terminal his-tag. The yield of our protein is very low in the soluble fraction, so we used this mAb to compare the soluble fraction to the inclusion body prep to see if there was a signal in the inclusion body prep indicating that our protein may not be soluble.
This antibody was used to capture his tagged proteins in various biosensor assays. This antibody works fine for general purposes. In some platforms (i.e., Octet), it is not as robust as a capture reagent, but it is still acceptable.