His Tag Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM050
Detection  of  His Tag by Western Blot.
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (5)

His Tag Biotinylated Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. This antibody detects His tags localized at the amino- or carboxyl-terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of  His Tag by Western Blot. View Larger

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine

Product Datasheets

Citations for His Tag Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Antibodies Targeting Closely Adjacent or Minimally Overlapping Epitopes Can Displace One Another
    Authors: YN Abdiche, AY Yeung, I Ni, D Stone, A Miles, W Morishige, A Rossi, P Strop
    PLoS ONE, 2017;12(1):e0169535.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  2. High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity.
    Authors: Abdiche, Yasmina, Miles, Adam, Eckman, Josh, Foletti, Davide, Van Blarcom, Thomas J, Yeung, Yik Andy, Pons, Jaume, Rajpal, Arvind
    PLoS ONE, 2014;9(3):e92451.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  3. Designed ankyrin repeat proteins: a new approach to mimic complex antigens for diagnostic purposes?
    Authors: Hausammann, Stefanie, Vogel, Monique, Kremer Hovinga, Johanna, Lacroix-Desmazes, Sebastie, Stadler, Beda M, Horn, Michael
    PLoS ONE, 2013;8(4):e60688.
    Species: Human
    Sample Types:
    Applications: Binding Assay
  4. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
    Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
    Int. J. Cancer, 2006;119(2):349-58.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen

FAQs

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Reviews for His Tag Biotinylated Antibody

Average Rating: 4 (Based on 5 Reviews)

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His Tag Biotinylated Antibody
By Anonymous on 06/20/2018
Application: Simple Western Sample Tested: Purified protein Species: Human

His Tag Biotinylated Antibody
By Anonymous on 12/01/2017
Application: Affinity Purification Sample Tested: antigen specific B cells Species: Rabbit

His Tag Biotinylated Antibody
By Anonymous on 05/16/2016
Application: WB Sample Tested: Serum Species: Mouse

His Tag Biotinylated Antibody
By Anonymous on 02/23/2016
Application: WB Sample Tested: Cell Lysates Species: E. coli

Antibody was used to probe cell lysates from E. coli to identify protein of interest which carries a C-terminal his-tag. The yield of our protein is very low in the soluble fraction, so we used this mAb to compare the soluble fraction to the inclusion body prep to see if there was a signal in the inclusion body prep indicating that our protein may not be soluble.


His Tag Biotinylated Antibody
By Anonymous on 02/22/2016
Application: biosensor capture assay. Sample Tested: Monoclonal Mouse IgG1 Clone # AD1.1.10 Species: Mouse

This antibody was used to capture his tagged proteins in various biosensor assays. This antibody works fine for general purposes. In some platforms (i.e., Octet), it is not as robust as a capture reagent, but it is still acceptable.