HIST1H3A Antibody (JU43-26)
Novus Biologicals | Catalog # NBP2-75522
Recombinant Monoclonal Antibody
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0008.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JU43-26 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human HIST1H3A aa 1-50 / 136 (acetyl K14). (SwissProt: P68431 Human; SwissProt: P68433 Mouse; SwissProt: Q6LED0 Rat)
Localization
Nucleus. Chromosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HIST1H3A Antibody (JU43-26)
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Western blot analysis of HIST1H3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate![Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0003.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0005.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded rat brain tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0006.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on Jurkat cell lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0007.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on rat liver (1) and mouse lung (2) tissue lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0001.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0002.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.rndsystems.com/images/products/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0004.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded human kidney tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.Applications for HIST1H3A Antibody (JU43-26)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Documents for HIST1H3A Antibody (JU43-26)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIST1H3A Antibody (JU43-26)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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