HIST1H3A [p Ser10] Antibody

Novus Biologicals | Catalog # NB100-97852

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Primate (100%), Rabbit (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HIST1H3A Primary Antibodies »

Product Specifications

Immunogen

Immunogen for A301-844A wthis antibody a synthetic phosphorylated peptide, which represented a portion of human Histone H3 surrounding serine 10 according to the numbering given in entry NP_003520.1 (GeneID 8350).

Reactivity Notes

D. melanogaster, Orangutan (100%).

Modification

p Ser10

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIST1H3A [p Ser10] Antibody

Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852]

Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852]

Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852]

Immunocytochemistry/ Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852]

Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.
Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852]

Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852]

Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB
Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852]

Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852]

Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.

Applications for HIST1H3A [p Ser10] Antibody

Application
Recommended Usage

Flow Cytometry

0.4 ug

Immunocytochemistry/ Immunofluorescence

1:2000 - 1:10000

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Western Blot

1:5000-1:10000
Application Notes
Flow Cytometry 0.4 ug per 1 million cells in a 150 mcl reaction. Immunofluorescence (ICC) 1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining. Immunohistochemistry 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HIST1H3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Alternate Names

H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Entrez Gene IDs

8350 (Human)

Gene Symbol

H3C1

UniProt

NP_003520.1 (Human)

Additional HIST1H3A Products

Product Documents for HIST1H3A [p Ser10] Antibody

Certificate of Analysis

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Product Specific Notices for HIST1H3A [p Ser10] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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