HIST1H3A Antibody

Name: HIST1H3A Antibody
Brand: Novus Biologicals
SKU: NBP2-16837
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-16837

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys9/Lys14 of human HIST1H3A. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIST1H3A Antibody

Western Blot: HIST1H3A Antibody [NBP2-16837]

Western Blot: HIST1H3A Antibody [NBP2-16837] - Histone H3 (acetyl Lys 9, Lys14) antibody detects Histone H3 (acetyl Lys 9, Lys14) protein by western blot analysis, 15% SDS-PAGE.

A. 30 ug HeLa whole cell lysate/extract (untreated)
B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin treatment for 18 hr)
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HIST1H3A Antibody [NBP2-16837] -

Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody (NBP2-16837) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Dot Blot: HIST1H3A Antibody [NBP2-16837] -

Dotblot analysis of anti-Histone H3 (acetyl Lys 9, Lys14) antibody with peptide samples.
Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys 9, Lys14) antibody (NBP2-16837) at different dilution as indicated.
A: Peptide samples of Histone H3.1
B: Peptide samples of Histone H3 (acetyl Lys 9, Lys14)

Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody [NBP2-16837] -

Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9K14ac (acetyl Lys9/Lys14) protein stained by Histone H3K9K14ac (acetyl Lys9/Lys14) antibody (NBP2-16837) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.

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Applications for HIST1H3A Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIST1H3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Alternate Names

H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Gene Symbol

H3C1

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Certificate of Analysis

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Product Specific Notices for HIST1H3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

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