Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free

Novus Biologicals | Catalog # NBP3-21528

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1513 expressed in HEK293

Format

BSA Free
View all Histone H1.4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-Histone H1.4 [Thr17] (Uniprot #: P10412)

Modification

p Thr 17

Specificity

Detects endogenous levels of total phospho-Histone H1.4 [Thr17]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free

Immunohistochemistry: Histone H1.4 [p Thr 17] Antibody (SR1513) [NBP3-21528] -

Immunohistochemistry: Histone H1.4 [p Thr 17] Antibody (SR1513) [NBP3-21528] - Immunohistochemical analysis of paraffin-embedded mouse colon, using NBP3-21528.

Western Blot: Histone H1.4 [p Thr 17] Antibody (SR1513) [NBP3-21528] -

Western Blot: Histone H1.4 [p Thr 17] Antibody (SR1513) [NBP3-21528] - Western blot analysis using NBP3-21528 in Jurkat cell lysate.

Applications for Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H1.4

The HIST1H1E gene encodes a 219 amino acid long, 21 kDA Histone H1.4 that functions by binding to linker DNA between nucleosome creating the macromolecular structure chromatin fiber. H1 histones as especially critical in condensation of nucleosome chains and the monitoring of individual gene transcription. HIST1H1E participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification. It interacts with genes HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4L. HIST1H1E has been researched regarding its role in progressive supranuclear palsy, pancreatitis, hepatitis, and x inactivation.

Alternate Names

dJ221C16.5, H1 histone family, member 4, H1.4, H1F4H1E, histone 1, H1e, histone cluster 1, H1e, histone H1.4, Histone H1b, MGC116819

Gene Symbol

H1-4

Additional Histone H1.4 Products

Product Documents for Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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