Histone H2a [ac Lys5] Antibody (GT1262) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13534

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # GT1262

Format

Azide and BSA Free
View all Histone H2A Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human Histone H2a. The exact sequence is proprietary.

Modification

ac Lys 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Histone H2a [ac Lys5] Antibody (GT1262) - Azide and BSA Free

Western Blot: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2a (acetyl Lys5) protein stained by Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:300. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Histone H2a [ac Lys5] Antibody (GT1262) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2A

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Long Name

H2A Histone Family

Alternate Names

H2A1B, HIST1H2AB

Gene Symbol

H2AC20

Additional Histone H2A Products

Product Documents for Histone H2a [ac Lys5] Antibody (GT1262) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2a [ac Lys5] Antibody (GT1262) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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FAQs

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