Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Novus Biologicals | Catalog # NBP3-33534

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2J2Q7
View all Histone H2AE Primary Antibodies »

Product Specifications

Immunogen

A synthetic hydroxylated peptide around Y39 of human Histone H2AE (P04908).

Sequence:
KGNYS

Modification

Hydroxy Tyr 39

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Western Blot: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] -

Western Blot: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Western blot analysis of various lysates using Histone H2AE Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Western Blot: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] -

Western Blot: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Western blot analysis of various lysates using Histone H2AE Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] -

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded human small intestine tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] -

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded rat colon tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] -

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded rat brain tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] -

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded mouse colon tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] -

Immunohistochemistry: Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7) [NBP3-33534] - Immunohistochemistry analysis of Histone H2AE in paraffin-embedded human esophagus tissue using Histone H2AE Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2AE

Histone H2AE is 124 amino acids long, weighing approximately 13 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes and is a core component of the nucleosome. Current studies are being done on several diseases and disorders including riddle syndrome, systemic lupus erythematosus, lupus srythematosus, and immunodeficiency. This protein has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the proteasome degradation, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Alternate Names

H2A histone family, member M, H2A/m, H2AFA, H2AFMHistone H2A/m, HIST1H2AE, histone 1, H2ab, histone cluster 1, H2ab, histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a

Gene Symbol

H2AC8

Additional Histone H2AE Products

Product Documents for Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

Certificate of Analysis

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Product Specific Notices for Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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