Histone H2A.Z Antibody (RM215) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-26045
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Vertebrate
Applications
Multiplex Immunofluorescence, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM215 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
A peptide corresponding to the C-terminus of Human Histone H2A.Z
Specificity
This antibody reacts to Histone H2A.Z protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2A.Z Antibody (RM215) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Histone H2A.Z Antibody (RM215) - Azide and BSA Free [NBP3-26045] -
Immunocytochemistry/ Immunofluorescence: Histone H2A.Z Antibody (RM215) - Azide and BSA Free [NBP3-26045] - Immunocytochemistry of HeLa cells, using Clone RM215 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Western Blot: Histone H2A.Z Antibody (RM215) - Azide and BSA Free [NBP3-26045] -
Western Blot: Histone H2A.Z Antibody (RM215) - Azide and BSA Free [NBP3-26045] - Western Blot of HeLa, HEK293, A375, SK-MEL-2 and A431 whole cell lysates, using RM215 at 0.5 ug/mL, showed endogenous Histone H2AZ in HeLa, HEK293, A375, SK-MEL-2 and A431 cells.
Immunocytochemistry/ Immunofluorescence: Histone H2A.Z Antibody (RM215) - Azide and BSA Free [NBP3-26045] -
Immunocytochemistry/ Immunofluorescence: Histone H2A.Z Antibody (RM215) - Azide and BSA Free [NBP3-26045] - Immunocytochemistry of HeLa cells, using Clone RM215 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H2A.Z Antibody (RM215) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.1 ug/ml - 1 ug/ml
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Western Blot
0.1 ug/ml - 0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2A.Z
Alternate Names
H2A histone family, member Z, H2A.Z, H2A/z, H2AZ histone, H2AZMGC117173, histone H2A.Z
Gene Symbol
H2AZ1
Additional Histone H2A.Z Products
Product Documents for Histone H2A.Z Antibody (RM215) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H2A.Z Antibody (RM215) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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