Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

Novus Biologicals | Catalog # NBP2-54770

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, S. pombe, Yeast

Cited:

Yeast

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B3F12/A9

Format

BSA Free
View all Histone H2B Primary Antibodies »

Product Specifications

Immunogen

Ub-polypeptide antigen based on amino acids 115 - 130 of yeast Histone H2B

Reactivity Notes

Saccharomyces cerevisiae

Modification

ubiquitylated Lys123

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Clone formerly called "H2BK123"

Scientific Data Images for Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]
Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]
ELISA: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

Applications for Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Yeast histone preparation.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2B

C-terminus monoubiquitination of histone H2B acts as a signal for downstream processes such as gene regulation, genome packaging, histone modification crosstalk, and DNA repair. How the ubiquitination levels are controlled and how the Ub signal is translated to downstream effects is poorly understood. An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B. The antibody is developed using an ubiquitin-polypeptide antigen based on the amino-acids 115-130 of the yeast H2B protein, linked by a proteolytically stable bond.

Long Name

H2B Histone Family

Alternate Names

HIST1H2BK

Gene Symbol

H2BC18

Additional Histone H2B Products

Product Documents for Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) - BSA Free

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Protocols

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