Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-26014

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Vertebrate

Applications

Multiplex Immunofluorescence, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM186 expressed in HEK293

Format

Azide and BSA Free
View all Histone H3 Primary Antibodies »

Product Specifications

Immunogen

This Histone H3 [Unmodified Lys4] antibody (RM186) was raised against an unmodified peptide corresponding to the N-terminus of Histone H3

Specificity

This Histone H3 [Unmodified Lys4] antibody (RM186) recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] -

Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of Histone H3 unmodified at Lys4.
Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] -

Immunocytochemistry/ Immunofluorescence: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Immunocytochemical staining of HeLa cells using Clone RM186 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

Multiplex Immunoassay: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP3-26014] -

Multiplex Immunoassay: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP3-26014] - RM186 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in Histone H3.

Applications for Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.5 ug/mL - 1 ug/mL

Immunocytochemistry/ Immunofluorescence

0.5 ug/ml - 2 ug/ml

Western Blot

1 ug/ml - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H3

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K,...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Alternate Names

H3F3A

Gene Symbol

H3C14

Additional Histone H3 Products

Product Documents for Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Interferons
Notch Pathway

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Protocols

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