Histone H3.1 [Monomethyl Lys18] Antibody (2B5) - BSA Free

Novus Biologicals | Catalog # NBP3-26134

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2B5 expressed in HEK293

Format

BSA Free
View all Histone H3.1 Primary Antibodies »

Product Specifications

Immunogen

Synthesized peptide [UniProt P68431]

Modification

Monomethyl Lys 18

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3.1 [Monomethyl Lys18] Antibody (2B5) - BSA Free

Histone H3.1 [Monomethyl Lys18] Antibody (2B5)

Western Blot: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] -

Western Blot: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Positive Western Blot detected in MCF-7 whole cell lysate. Hela whole cell lysate. HepG2 whole cell lysate. SH-SY5Y whole cell lysate, Rat brain tissue.
All lanes: Histone H3.1 [Monomethyl Lys18] Antibody at 0.7ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 15 KDa
Observed band size: 15 KDa
Histone H3.1 [Monomethyl Lys18] Antibody (2B5)

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Analysis of Histone H3.1 [Monomethyl Lys18] Antibody (2B5) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Histone H3.1 [Monomethyl Lys18] Antibody (2B5)

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Staining of Hela cells with Histone H3.1 [Monomethyl Lys18] Antibody (2B5) at 1:43, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Histone H3.1 [Monomethyl Lys18] Antibody (2B5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Histone H3.1

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser 10 and Ser 28, both of which are extensively phosphorylated in DNA-bound forms of Histone H3 and in nucleosomal Histone H3. The nucleosome structure of Histone H3 promotes N-terminal phosphorylation in vitro. Phosphorylation of Histone H3 at Ser 10 is an essenti

Long Name

Histone Cluster 1 H3 Family Member A

Alternate Names

HIST1H3A

Gene Symbol

H3C6

Additional Histone H3.1 Products

Product Documents for Histone H3.1 [Monomethyl Lys18] Antibody (2B5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H3.1 [Monomethyl Lys18] Antibody (2B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Notch Pathway

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Protocols

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