Histone H3.1 Antibody

Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NBP2-75524]

Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NBP2-75524]

Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: Histone H3.1 Antibody [NBP2-75524] -

Abnormal H3K9me3 is incidental to incorrect H3.1/3.2 incorporation introduced by major satellite RNA.a Immunostaining for H3.1/3.2 and H3.3 in representative pronucleus of Ctrl and MajSat_OE zygotes. Scale bars, 20 um. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. b Scatterplot showing H3.1/3.2 and H3.3 fluorescence intensity mean values in pronucleus of Ctrl and MajSat_OE zygotes. The statistical data are expressed as mean +/- SEM, *p < 0.05 and ns means not significant (H3.1/3.2: male p = 0.0438, female p = 0.6590, H3.3: male p = 0.0380, female p = 0.5885) by two-sided Student’s t test for each comparison. Each treatment contains 3 biological replicates, with each replicate containing at least 5 zygotes. c Immunostaining for H3K14ac in representative pronucleus of Ctrl and Lsm1_KD zygote. Scale bars, 20 um. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. d Immunostaining for H3K14ac in representative pronucleus of Ctrl and MajSat_OE zygote. Scale bars, 20 um. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. e Scheme for the RNA pulldown to determine the interaction between MajSat RNA and H3.1/H3.3. f, g Pulldown of H3.1, H3.3 using intact and truncated MajSat RNA. Representative images from 3 biological replicates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36810573), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Histone H3.1 Antibody [NBP2-75524] -

Abnormal H3K9me3 is incidental to incorrect H3.1/3.2 incorporation introduced by major satellite RNA.a Immunostaining for H3.1/3.2 and H3.3 in representative pronucleus of Ctrl and MajSat_OE zygotes. Scale bars, 20 um. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. b Scatterplot showing H3.1/3.2 and H3.3 fluorescence intensity mean values in pronucleus of Ctrl and MajSat_OE zygotes. The statistical data are expressed as mean +/- SEM, *p < 0.05 and ns means not significant (H3.1/3.2: male p = 0.0438, female p = 0.6590, H3.3: male p = 0.0380, female p = 0.5885) by two-sided Student’s t test for each comparison. Each treatment contains 3 biological replicates, with each replicate containing at least 5 zygotes. c Immunostaining for H3K14ac in representative pronucleus of Ctrl and Lsm1_KD zygote. Scale bars, 20 um. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. d Immunostaining for H3K14ac in representative pronucleus of Ctrl and MajSat_OE zygote. Scale bars, 20 um. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. e Scheme for the RNA pulldown to determine the interaction between MajSat RNA and H3.1/H3.3. f, g Pulldown of H3.1, H3.3 using intact and truncated MajSat RNA. Representative images from 3 biological replicates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36810573), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Histone H3.1 Antibody [NBP2-75524] -

APCDD1L-AS1 overexpression induced histones expression disorders. A The overexpression efficiency of APCDD1L-AS1 in OSRC2 cells. B The TMT results showed that 39 proteins were downregulated and 66 proteins were upregulated in APCDD1L-AS1 overexpressed OSRC2 cells compared with its control cells. C Biological Process GO term enrichment analysis results of the complete 105 statistically significant proteins. D The TMT results of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2. E The protein expression of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2 in the same cell protein samples. F The protein expression of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2 in the in mice tumors. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35414787), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Histone H3.1 Antibody [NBP2-75524] -

APCDD1L-AS1 overexpression induced histones expression disorders. A The overexpression efficiency of APCDD1L-AS1 in OSRC2 cells. B The TMT results showed that 39 proteins were downregulated and 66 proteins were upregulated in APCDD1L-AS1 overexpressed OSRC2 cells compared with its control cells. C Biological Process GO term enrichment analysis results of the complete 105 statistically significant proteins. D The TMT results of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2. E The protein expression of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2 in the same cell protein samples. F The protein expression of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2 in the in mice tumors. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35414787), licensed under a CC-BY license. Not internally tested by Novus Biologicals.